Fig. 5.

Transcriptional regulation of Hif-1α by Meis1. a) Real Time PCR of Meis1 in low and high MP cells demonstrates significantly higher levels of Meis1 expression in low MP cells. b) Upregulation of Meis1 in Kasumi-1 cells compared to human MPB Cells and c) Upregulation of Hif-1α in Kasumi-1 cells compared to human MPB Cells. d) Real time PCR of Meis1 and Hif-1α in Kasumi-1 cells following siRNA knockdown of Meis1. e) Real time PCR of lactate dehydrogenase (LDHA), Glut1 (SLC2A1) and phosphofructokinase (PFKL) following siRNA knockdown of Meis1. Note the significant downregulation of Hif-1α mRNA and Hif-1α target genes following siRNA knockdown of Meis1. f) Relative metabolic shift towards increased oxidative phosphorylation in Kasumi-1 cells following Meis1 knockdown: There is increased oxygen consumption and ATP levels following knockdown of Meis1 in Kasumi-1 cells. n = 3,*p < 0.05, **p < 0.01