Table III.

5HLDS(15)BA(Y) redirected T cell cytotoxicity of human tumor cell lines

Tumor type	Cell lines	GD2 expression (MFI)	Maximum level of lysis	Tumor cytotoxicity (EC50)(nM)
Melanoma	SKMEL-1	38	36%	0.01
Neuroblastoma	LAN-1	201	52%	0.04
Neuroblastoma	NMB-7	127	60%	0.05
Melanoma	M14	50	50%	0.09
Neuroblastoma	BE(1)N	94	46%	0.17
Neuroblastoma	IMR-32	128	58%	0.20
Melanoma	HT-144	13	35%	0.48
Small cell lung cancer	NCI-H524	66	58%	0.61
Neuroblastoma	SKNJC2	6	25%	0.86*
Melanoma	SKMEL28	8	28%	2.20*
small cell lung cancer	NCI-H69	27	39%	3.16*
Breast Cancer	MDA-MB-468	6	13%	3.89*
small cell lung cancer	NCI-H196	8	7%	> 185
small cell lung cancer	NCI-H345	7	2%	>185

GD2 expression of cell lines were expressed as mean fluoresce intensity. Cytotoxicity assays (⁵¹Cr release) were carried out with cultured T cells at E: T ratio of 10:1. Maximum level of lysis was measured at a 18.5 nM concentration of 5HLDS(15)BA (Y). EC50 was calculated using Sigma plot 8.0.

^*Numbers were extrapolated by Sigma plot 8.0.