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Stimulation of mouse splenocytes proliferation by LPS and/or CD40L. Cultured mouse slpenocytes were treated with E. coli LPS (10 µg/ml) and/or CD40L (1 µg/ml) for 2 days and the overall cell proliferation status was determined by CellTiter 96 AQueous Assay (Promega Corp). The results are representative of at least six independent experiments in duplicates. Data are presented as mean ± SEM, n = 6. Student t-test, *P < 0.05, **P < 0.01.
