Table. Primers used in study of the detection and full-length genome characterization of novel canine vesiviruses.

Primer	Position*	Sequence	Sense	Use†
493F	5127–5150	GGT TTG CCA TCT GGC ATG CCG CTA	+	Detection
526R	5793–5816	AGC CAT VGC TCA RTT CTC AAA CAC	−	Detection
p289	5271–5292	TGA CAA TGT AAT CAT CAC CAT A	−	Detection
p290	4962–4984	GAT TAC TCC AAG TGG GAC TCC AC	+	Detection
501F	554–577	GTC TTG TGC TCT VTA CGA CAM ATG	+	Sequencing
VN3T20	3′- poly A	GAG TGA CCG CGG CCG CT20.	−	3′ RACE
502F	1215–1234	ATG ATW ATT GAH AAC CAY GA	+	Sequencing
509F	2904–2923	TAC GAT ATG GCY TGG GCY CT	+	Sequencing
510F	3411–3432	GAT GAT GAG TAC GAT GAR TGG A	+	Sequencing
511F	3588–3607	GAA GAC GTC ACC RTA ATT GG	+	Sequencing
513F	3876–3898	GTT ACG TTC RAT GGY GAA TTG GC	+	Sequencing
515F	4314–4338	CAC GTG TCA CCA GCA CAC RTD GAT G	+	Sequencing
504R	1599–1618	ACC ACG CTY TCR TTS GAC CA	−	Sequencing
528R	2887–2909	CTT GTC ATC TTA GTG TAC AAT GA	−	Sequencing
506R	1680–1702	AG GTT GGT RAC NGC RTC AAT GTC	−	Sequencing
508R	1974–1993	GT GTA GGC RYC GTG GTG GTC	−	Sequencing
512R	3588–3608	GCC AAT TAY GGT GAC RTC WTC	−	Sequencing
536F	714–732	TAC GAT CTT GCA ATC AAT G	+	Sequencing
GE72F	1915–1935	CCT ATG CCA TTG CGT CTA GAC	+	Sequencing
GE73R	2951–2970	CAG CCT TAA GTG CCT GCC AC	−	Sequencing
SEQ100	5634–5653	TGT CGC CAA ATG TTG ATG AG	+	Sequencing
SEQ101	6343–6360	TTG CCA CAG GCA CTC AGC	+	Sequencing
SEQ102	6860–6877	GGA AAC ACG TGG TGG TCA	+	Sequencing
SEQ103	7487–7504	AAG TAG AAT GAT TGG TGA	+	Sequencing
SEQ104	8063–8082	GAG TTT GAC AAG ATG AAC AG	+	Sequencing
GSP1	497–520	GCT TCA GAG ATC AGA ATA TCG TTG	−	5′ RACE
GSP2	366–385	GTG GTC AGA GCC TTG GTC AG	−	5′ RACE

*Position is based on the sequence of strain Bari/212/07/ITA (GenBank accession no. JN204722).
†RACE, rapid amplification of cDNA ends.