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. 2015 Jul 28;11(7):e1004974. doi: 10.1371/journal.ppat.1004974

Fig 11. RCA analysis suggests episomal persistence of MCVSyn genomes.

Fig 11

Inverted image of a ethidium bromide-stained agarose gel with input material (lanes 1,3,5,7 and 9) or RCA products (lanes 2,4,6,8, 10 and 11) from mock-transfected PFSK-1 cells (lanes 1 and 2), MCVSyn-transfected PFSK-1 cells at 4 days (lanes 3 and 4) or 136 days (lanes 9 and 10) post transfection, the MCC-derived cell lines WaGa or MKL-1 (lanes 5 and 6 or 7 and 8, respectively) or a water control (lane 11). DNA was subjected to restriction enzyme digestion to produce linear, unit-length viral genomes from concatameric RCA products. For size comparison, lane 12 shows unit length MCVSyn genomes excised from plasmid pMK-MCVSyn. The asterisk marks the position of the bacterial pMK plasmid backbone.