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. 2015 May 22;1(4):e1500169. doi: 10.1126/sciadv.1500169

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Copyright © 2015, The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.

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Fig. 1 — Time course of single-turnover GTP hydrolysis by wild-type (WT; black) and H91A (red) EF-G. The amount of [³H]GDP produced is plotted against time and fitted with a single exponential function. The rate constants are 202 ± 29 s⁻¹ for WT and 28 ± 4.5 s⁻¹ for H91A EF-G. Error bars represent SDs obtained from three independent experiments. Ribo., ribosome.