Table 1.
Reversion rates of ura3-104 in two orientations (OR1 and OR2) in the S288C yeast strain carrying pol3-L612M in combination with mutations in different mismatch removal processes
See also Figures S5 and S6.
| Strain | Ura+ rate [×10−9] (95% CI) | CAG rate [×10−9] (95% CI) | Numbers of CAG : numbers of GAG+TCG+TTG+AAG |
|---|---|---|---|
| OR2 (T:G on lagging strand) | |||
| WT | 2.5 (1.7–3.3) | 1.3 (0.9–1.7) | 47 : 48 |
| pol3L612M | 22 (16–28) | 21 (15–27) | 103 : 4 |
| pol3L612M pol2-4 | 20 (18–22) | 19 (17–21) | 122 : 8 |
| pol3L612M exo1 Δ | 140 (138–142) | 132 (130–134) | 116 : 7 |
| pol3L612M pol2-4 exo1 Δ | 440 (395–485) | 419 (376–462) | 120 : 6 |
| pol3L612M msh2 Δ | 399 (303–495) | 396 (301–491) | 117 : 1 |
| pol3L612M msh2Δ exo1Δ | 488 (444–532) | 488 (444–532) | 124 : 0 |
| OR1 (T:G on leading strand) | |||
| WT | 2.7 (2–3.4) | 1.2 (0.9–1.4) | 35 : 47 |
| pol3L612M | 4.0 (3.8–5.2) | 2.4 (1.7–3.1) | 57 : 36 |
| pol3L612M pol2-4 | 3.5 (2.8–4.2) | 3.5 (2.8–4.2) | 121 : 0 |
| pol3L612M exo1 Δ | 22 (21–23) | 15 (14–16) | 84 : 48 |
| pol3L612M pol2-4 exo1 Δ | 206 (159–253) | 206 (159–253) | 123 : 0 |
| pol3L612M msh2 Δ | 917 (775–1059) | 896 (758–1034) | 124 : 2 |
| pol3L612M msh2Δ exo1 Δ | 1172 (942–1420) | 1143 (901–1385) | 118 : 3 |