Fig 10. Effect of 2C^ATPase helicase-defective GK134AA mutation on EV71 RNA synthesis and viability.

(A) Wt and GK134AA mutant EV71 RNA transcripts were transfected into human RD cells. The total RNA from differently transfected cells were exacted, and the levels of EV71 RNA were measured via qRT-PCR 24 and 48 hours post-transfection (h.p.t). For either Wt or mutant EV71 transcript, the relative EV71 RNA production 48 h.p.t. was determined by comparing the RNA product level at 48 h.p.t. with that at 24 h.p.t. Error bars represent SD, n = 3. (B) The virus production of either Wt (upper panel) or GK134AA mutant (lower panel) EV71 transcript in RD cells 24 h.p.t. was detected via immunofluorescent staining of EV71 VP1 with anti-VP1 antibody (green). The cell nuclei were stained with DAPI (blue). The merged image represents the digital superimposition of green and blue signals.