Table 1. Cytokine production by splenocytes stimulated with inactivated bacteria of different Bifidobacterium strains.
Control/ Bifidobacterium strain | IL-10 | TNF-α | IL-6 | IFN-γ | |
---|---|---|---|---|---|
Medium | 7 ± 1 | 6 ± 3 | 13 ± 4 | 12 ± 11 | |
PAM3C | 628 ± 57** | 166 ± 20 | 175 ± 84** | 790 ± 332 | |
B. longum | Bl 7952 | 270 ± 25** | 138 ± 12 | 452 ± 35 | 173 ± 80 |
B. longum | Bl 372 | 532 ± 39** | 451 ± 37** | 638 ± 80** | 744 ± 183 |
B. infantis | Bi 369 | 542 ± 85** | 384 ± 56** | 493 ± 117 | 108 ± 59 |
B. animalis | Ban 218 | 187 ± 14** | 935 ± 198** | 650 ± 62** | 1865 ± 422** |
B. animalis | Ban 366 | 268 ± 14** | 200 ± 22 | 229 ± 13 | 55 ± 24 |
B. adolescentis | Bad 368 | 92 ± 4 | 189 ± 17 | 334 ± 52 | 154 ± 91 |
B. adolescentis | Bad 370 | 243 ± 16** | 357 ± 36** | 923 ± 114** | 669 ± 151* |
B. adolescentis | Bad 371 | 606 ± 52** | 290 ± 8* | 675 ± 70** | 145 ± 65 |
B. adolescentis | Bad 373 | 186 ± 14* | 495 ± 60** | 987 ± 87** | 1819 ± 275** |
Splenocytes isolated from naïve mice (n = 5) were stimulated with formalin-inactivated bifidobacteria (6 x 107 CFU/ml) for 48 h. Pam3CSK4 (PAM3C, 1μg/ml) was use as a positive control. Non-stimulated splenocytes (Medium) were evaluated as control of basal cytokine levels. Concentration of cytokines in supernatants was determined by multiplex assay. Data are expressed as mean ± SEM. Results are representatives of two repeat experiments. Significant difference to medium was calculated using One-way ANOVA and Dunnett’s multiple comparison post-hoc test *p < 0.05; **p < 0.01.