Table 1. Cytokine production by splenocytes stimulated with inactivated bacteria of different Bifidobacterium strains.

Control/ Bifidobacterium strain		IL-10	TNF-α	IL-6	IFN-γ
Medium		7 ± 1	6 ± 3	13 ± 4	12 ± 11
PAM3C		628 ± 57**	166 ± 20	175 ± 84**	790 ± 332
B. longum	Bl 7952	270 ± 25**	138 ± 12	452 ± 35	173 ± 80
B. longum	Bl 372	532 ± 39**	451 ± 37**	638 ± 80**	744 ± 183
B. infantis	Bi 369	542 ± 85**	384 ± 56**	493 ± 117	108 ± 59
B. animalis	Ban 218	187 ± 14**	935 ± 198**	650 ± 62**	1865 ± 422**
B. animalis	Ban 366	268 ± 14**	200 ± 22	229 ± 13	55 ± 24
B. adolescentis	Bad 368	92 ± 4	189 ± 17	334 ± 52	154 ± 91
B. adolescentis	Bad 370	243 ± 16**	357 ± 36**	923 ± 114**	669 ± 151*
B. adolescentis	Bad 371	606 ± 52**	290 ± 8*	675 ± 70**	145 ± 65
B. adolescentis	Bad 373	186 ± 14*	495 ± 60**	987 ± 87**	1819 ± 275**

Splenocytes isolated from naïve mice (n = 5) were stimulated with formalin-inactivated bifidobacteria (6 x 10⁷ CFU/ml) for 48 h. Pam3CSK4 (PAM3C, 1μg/ml) was use as a positive control. Non-stimulated splenocytes (Medium) were evaluated as control of basal cytokine levels. Concentration of cytokines in supernatants was determined by multiplex assay. Data are expressed as mean ± SEM. Results are representatives of two repeat experiments. Significant difference to medium was calculated using One-way ANOVA and Dunnett’s multiple comparison post-hoc test *p < 0.05; **p < 0.01.