Skip to main content
. 2015 Jul 13;29(8):1114–1122. doi: 10.1210/ME.2014-1181

Figure 2.

Figure 2.

Comparison of AA activation ERK1/2 and mTORC1 in MIN6 cells. A, Stimulation with Ala (lanes 1–5) or Ser (lanes 7–11) at 0.05, 0.16, 0.5, 1, or 5 mM for 2 minutes or unstimulated (lane 6). One of four (for Ser) or three (for Ala) experiments are shown. B, Stimulation with 0.25, 0.5, 1, or 5 mM Ala or Ser for 30 minutes. Top panel, Immunoblots for one of three experiments. Bottom panel, Quantification (n = 3). *, P < .05, **, P < .01, using one-way ANOVA with Fisher's posttest. Stimulation of cells with 0.5 mM Gln, EAA, or NEAA, with or without Gln for 2 minutes (C) and 30 minutes (D), which is representative of four (C) and six (D) experiments. Cells were preincubated for 1 hour without or with 1 mM Gln (Gln loaded) with the indicated dilutions of the AA mixture for 2 minutes (E) or 30 minutes (F), which is representative of three (E) and six (F) independent experiments. Numbers under blots are normalized to total ERK1/2 and fold change relative to the control as in Figure 1 (C and E) or S6 (D and F). Gln (1- to 7-fold), EAA (4- to 25-fold), NEAA (13- to 147-fold), Gln/EAA (6- to 31-fold), and Gln/NEAA (3- to 188-fold).