Figure 8.

Androgen-sensitive adaptors modulate androgen-dependent proliferation of prostate tumor cells. A, Twenty-four-hour AD LNCaP cells were transfected with control or experimental siRNAs (100nM) for 24 hours. Vehicle (ethanol, 0nM R1881) or androgen (0.01nM or 0.1nM R1881) was then added to the cells, and they were incubated for a further 120 hours. The relative cell number was determined using the CyQUANT Cell Proliferation Assay. ANOVA was only performed between control and target siRNA treated with the same dose of androgen. Asterisks denote statistical significance (ANOVA; *, P < .05, n = 6). B, Heatmap presentation of proliferation assay results. Significant differences between cells transfected with experimental siRNAs and control siRNAs were color coded as indicated in the legend.