Dependence of hydrogel shear moduli and thixotropy on crosslinking strength and molecular weight between crosslinks. (a) Binding isotherms obtained from isothermal titration calorimetry (ITC) injections of C1 peptide into P1 or P2. Table shows binding enthalpy and stoichiometry derived from fitting binding isotherms to an independent site model. (b) Storage moduli (G’, closed symbols) and loss moduli (G”, open symbols) of MITCH-PEG variants as a function of frequency (10% w/v gels, 5% strain, 37 °C). (c) Linear shear viscosity measured under alternating shear rates, showing shear-thinning and self-healing behavior (10% w/v gels, 30-sec durations of 0.1 and 10 s−1 shear rate, 37 °C).