Figure 6.

SirTM Function Is Linked to Oxidative Stress Responses

(A) Wild-type C. albicans and mfs1Δ mutants were inoculated onto plates with or without 5 mM H₂O₂ and growth assayed after 2 days of incubation at 30°C. Four independent mfs1 homozygous deletion clones (labeled #1–#4) were tested. Moderate protection from oxidative stress was observed for all four mutant clones.

(B) The extent of growth inhibition by H₂O₂ was quantified in 96-well plate-based broth assays (see Supplemental Experimental Procedures). Following a 24 hr incubation at 37°C, percentage of growth was calculated relative to control (no H₂O₂). The experiment was performed independently twice, in biological duplicates (two independent colonies per strain). A similar trend was observed in both experiments. Shown are averages of growth inhibition ± SD for one of the experiments.

See also Figure S7.