Fig. 5.

Multiblock C-PCA of GC-TOF/MS metabolite profiles. (a) C-PCA scores plot (six biological replicates per class), the data were divided into two blocks corresponding to presence (+) and absence (−) of nitrate; the total explained variance (TEV) for each PC is given. Leaf base (B red circle), mid leaf (M green circle) and leaf tip (T blue circle). (b) C-PCA loadings plot corresponding to (a). Variables with loadings scores within the ±0.1 threshold are labelled, index numbers correspond to Table S1. (c) C-PCA scores plot based upon six biological replicates for each of the six experimental classes, the data were divided into three blocks corresponding to leaf base, mid leaf, and leaf tip sections. Labels indicate plants grown in the presence (N+ blue circle) and absence (N− red circle) of nitrate. (d) C-PCA loadings plot corresponding to (c). Variables with loadings scores within the ±0.1 threshold are labelled, index numbers correspond to Table S1.