Fig. 6.

Box and whisker trend plots of significant metabolites determined by GC-TOF/MS. Metabolite levels in leaf base (B), mid leaf (M) and leaf tip (T) sections from the developing primary wheat leaves of plants grown in the presence (+) or absence (−) of nitrate measured using GC-TOF/MS. The normalised peak areas representing the mean of each metabolite and experimental class are displayed as box and whisker plots. Within the plots, the box represents the interquartile range (25% and 75%), the whiskers (error bars) represent data points not considered as outliers defined by 1.5× the interquartile range deviation from the mean. In cases where the data distribution is very tight and values lie only just outside of the 25% or 75%, the whiskers may not be clearly visible due to overlap with the box.