Table 2.
Synergistic effect of β-catenin and Atoh1 on Lgr5+ cells' proliferation in the IHC regiona
| Genotype | No. of HA+/BrdU+ cells/300 μm (P4) | Sample size (n) |
|---|---|---|
| Lgr5CreER | ND | 3 |
| Lgr5CreER; β-catenin+ | ND | 3 |
| Lgr5CreER; HA+ | 0 | 3 |
| Lgr5CreER; β-catenin+; HA+ | 3.40 ± 0.59 | 5 |
a Coexpression of β-catenin and Atoh1-HA in Lgr5+ cells results in a synergistic proliferation response. Number of HA+/BrdU+ cells per 300 μm section at P4 after 4 consecutive injections (2 h apart) of BrdU are shown with mean ± SEM. ND, Not detected. N = 3–7 for each time point and each genotype.