Table 6.
Lack of synergistic effects on proliferation and differentiation of IPhCs/IBCsa
| Genotype | No. of HA+ cells/300 μm | Sample size (n) | Genotype | No. of HA+/Myo7a+ cells/300 μm | Sample size (n) |
|---|---|---|---|---|---|
| P8 | |||||
| PLP/CreERT/+; | ND | 3 | PLP/CreERT/+; | ND | 3 |
| PLP/CreERT/+; β-catenin | ND | 3 | PLP/CreERT/+; β-catenin | ND | 3 |
| PLP/CreERT/+; HA+ | 35.23 ± 10.05 | 3 (NS, p = 0.7223; df = 5) | PLP/CreERT/+; HA+ | 4.43 ± 2.15 | 3 (NS, p = 0.7013; df = 5) |
| PLP/CreERT/+; β-catenin; HA+ | 30.63 ± 7.54 | 4 | PLP/CreERT/+; β-catenin; HA+ | 5.98 ± 2.82 | 4 |
| P21 | |||||
| PLP/CreERT/+ | ND | 3 | PLP/CreERT/+ | ND | 3 |
| PLP/CreERT/+; β-catenin | ND | 3 | PLP/CreERT/+; β-catenin | ND | 3 |
| PLP/CreERT/+; HA+ | 36.62 ± 9.02 | 5 (NS, p = 0.9439; df = 9) | PLP/CreERT/+; HA+ | 23.40 ± 7.20 | 5 (NS, p = 0.4691; df = 9) |
| PLP/CreERT/+; β-catenin; HA+ | 35.70 ± 8.84 | 6 | PLP/CreERT/+; β-catenin; HA+ | 17.17 ± 4.59 | 6 |
a There is no synergistic effect on the proliferation or differentiation of the IPhCs/IBCs SCs at P8 or P21 in the presence of β-catenin and Atoh1. Statistical analysis of HA+ and HA+/Myo7a+ cell counts per 300 μm sections did not show any significant differences between PLP/CreERT+;Atoh1-HA+ and PLP/CreERT+;β-catenin; HA+ mice at P8 and P21. ND, Not detected; NS, not significant using unpaired Student's t test with Bonferroni correction. N = 3–6 for each time point and each genotype. Data are mean ± SEM.