Figure 3.

The C-terminal mitochondrial targeting sequence of NFAT1 is required for mitochondrial translocation. A, Schematic diagrams show GFP-tagged NFAT1 full-length (FL) and mutant expression vectors and their capability for mitochondrial targeting in microglia after treatment with LPSs for 24 h. B, Confocal images show expression of exogenous GFP-tagged NFAT1 (green) and endogenous COX1 (red) in NFAT1-transfected primary microglia treated with LPSs for 24 h. Line graphs illustrate the fluorescence intensities of GFP-NFAT1 (green) and COX1 staining (red) along the white dashed lines in the merged images. Scale bar, 10 μm.