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. 2015 Aug;43(8):1226–1235. doi: 10.1124/dmd.115.064428

Fig. 5.

Fig. 5.

RT-PCR analysis of minigene mRNA products. (A) Exon2-intron2-exon3 minigenes: two minigenes contained CYP2C19 exon 2 + intron 2 + exon 3 (differing only by rs12769205A>G) that replaced the BamH1/Xho1 fragment [minigene exon (Em)] of the RHCglo minigene. (B) Exon 5 minigenes: Two minigenes contained the last 87 nt of CYP2C19 intron 4 and all of exon 5 (differing only by rs4244285) that replaced the Sal1/Xho1 fragment of the RHCglo minigene. HepG2 cells were transfected with each of the four plasmids and minigene mRNA products were analyzed by RT-PCR using the RSV5U/TNIE4 primers and the products analyzed on agarose gels. The rs12769205 minigene generated a transcript with the exon 2B insertion (A), and the rs4244285 minigene generated a transcript with the 40 bp exon 5 deletion (B).