Fig. 1.

Schwann cell culture purity and CNPase expression. Cultures were probed with the Schwann cell markers S100 red (A) and CNPase green (B) to confirm the purity of the primary rat Schwann cell cultures (4′,6-diamidino-2-phenylindole, blue; scale bar = 50 μm). (C) Western blots for TNAP, CNPase, and α-tubulin on Schwann cell lysates from cultures grown in either growth or defined medium. Defined medium did not alter expression of either TNAP or α-tubulin, but increased expression of CNPase. (D) Quantification of CNPase expression between the cultures grown in growth medium versus defined medium (normalized to α-tubulin as a loading control) revealed a *significant increase in CNPase cultures grown in defined medium. Ab, antibody; CNP1, CNPase isoform type 1; CNP2, CNPase isoform type 2.