Fig. 9.

Effect of CM572 on Bid cleavage in SK-N-SH neuroblastoma. SK-N-SH cells were treated with 30 µM CM572 for the indicated times. Cells were then extracted and subjected to Western blotting for Bid as described in Materials and Methods. (A) A 30-µM treatment of CM572 induced cleavage of Bid as indicated by decreased levels of full-length Bid (22 kDa) with increasing incubation times. α/β-Tubulin (55 kDa) was used as a loading control. Experiment was repeated twice with similar results. A representative Western blot is shown. (B) Quantitation of Western blot data. The optical density (OD) ratios of Bid to tubulin were determined for each time point, and the ratio in the control was set to 1. Ratios are expressed relative to control ratio. Data shown are the average of two experiments.