Fig. 4.

Menthol modulation of α3β4 nAChRs is dependent on the concentration of ACh. (A) Representative whole-cell inward currents in response to different concentrations of ACh in the absence (left) and in the presence (right) of 300 μM menthol in α3β4 nAChR-expressing cells. The current decay during desensitization was best fit to a one- or two-exponential function, yielding the indicated time constants. (B) Mean weighted average time constants for decay and (C) percent of desensitization (D) of the ACh-mediated current obtained in the absence of menthol (open circle) and in the presence of 300 μM of menthol (filled circle). Data are mean ± S.E.M., n = 3–5.