Figure 5.

Western blot analysis to measure the effect of L-NAME treatment on the LC3, ATG7 and Beclin 1 expression in the hippocampus of rats. (A) 60 μg proteins were alienated on SDS-PAGE, western blotted, probed with specific primary antibodies, and reprobed with control loading antibody (One typical western blot of each antibody is shown: n = 6). (B) The densities of LC3І and LC3ІІ bands were evaluated and the LC3ІІ/LC3І ratio was measured. (C) The densities of ATG7 bands were evaluated and their ratios to β-actin were measured. (D) The densities of Beclin 1 bands were evaluated and their ratios to β-actin were measured. Each point shows the mean ± SEM. (*P < 0.05; **P < 0.01 different from the control group, #P < 0.05; ##P < 0.01 different from the Aβ-injected group