Figure 3.

Effects of IRS869 on the expression of TLR9 and its major downstream molecules in plaques. The expressions of TLR9, MyD88, p-NF-κB, and IRF7 were determined by western blotting. β-actin (for TLR9, MyD88, and IRF7) or NF-κB (for p-NF-κB) served as the loading control. The bars represent the mean ± SEM of three separate experiments. ^#<0.01 compared with normal control littermates, ^∗ P < 0.05 compared with ApoE^−/− saline controls. TLR9: toll-like receptor 9; MyD88: myeloid differentiation protein 88; NF-κB: nuclear factor-kappa B; p-NF-κB: phosphorylated NF-κB; IRF7: interferon regulatory factor 7.