Figure 5.

Effects of ODN1826 and IRS869, alone or in combination, on the percentages of M1 and M2 subtype in RAW264.7 macrophages. Representative flow cytometry analyses of M1 and M2 cells and double immunofluorescence labeling of iNOS and CD206 macrophages in RAW264.7 cells. RAW264.7 cells were treated for 24 h with 5 μM ODN1826 (a, e), 5 μM IRS869 (b, f), ODN1826+IRS869 (c, g), or vehicle (d). The cells were stained with PE-conjugated anti-CD206 (red) and FITC-conjugated anti-iNOS (green) monoclonal antibodies. Nuclei were stained with DAPI (blue).