Table 1. Quantification of ETC complexes and supercomplexes in wild-type, ΔPaRcf1 and ΔPaRcf2 mitochondria.
| (AU) | Wild type | ΔPaRcf1 | ΔPaRcf2 |
|---|---|---|---|
| I1III2IV2 (S2) | 1.00 ± 0.20 | 0.07 ± 0.01 | 0.95 ± 0.33 |
| I1III2IV1 (S1) | 1.00 ± 0.08 | 0.03 ± 0.02 | 1.11 ± 0.18 |
| I1III2IV0 (S0) | 1.00 ± 0.38 | 15.30 ± 2.58 | 1.16 ± 0.16 |
| V2 | 1.00 ± 0.13 | 1.00 ± 0.08 | 1.10 ± 0.08 |
| I1 | 1.00 ± 0.29 | 1.33 ± 0.18 | 1.36 ± 0.18 |
| V1/III2 | 1.00 ± 0.13 | 1.12 ± 0.12 | 1.15 ± 0.04 |
| IV1 | 1.00 ± 0.43 | – | 1.43 ± 0.42 |
| (n = 4) | (n = 3) | (n = 4) |
Quantitative BN-PAGE analysis of ETC complexes and supercomplexes in mitochondrial protein extracts from wild type (n = 4), ΔPaRcf1 (n = 3) and ΔPaRcf2 (n = 4). Densitometric quantification after Coomassie staining of BN gels was performed with the image processing and analysis software ‘ImageJ’ according to the developer’s documentation. Optical densities of the different complexes and supercomplexes were normalized to total Coomassie staining of the corresponding lane. The mean wild-type abundances were defined as 1. Data are mean protein abundance ± s.e.m. in arbitrary units (AU).