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. 2015 Jul 29;5:12723. doi: 10.1038/srep12723

Figure 5. Specificity evaluation of PSR assay.

Figure 5

(A): Real-time turbidimeter instrument recorded the PSR amplification plot of different clinical strains. Amplification was performed at 65 °C for 60 min. (B): PCR detection of blaNDM-1 in different strains. The product sequence is 813 bp. (C): Visual results of PSR method under UV light. 1, Escherichia coli pGEX-NDM-BL21; 2, S. maltophilia JKYJ-01; 3, E. faecalis PIJ; 4, A. baumannii H949; 5, A. baumannii B260; 6, P. aeruginosa D104; 7, S. marcescens SY-67; 8, S. sonnei 2531; 9, S. flexneri 4536; 10, S. entericasero type Paratyphi 86423; 11, enteroinvasive E. coli 44825; 12, enterotoxigenic E. coli 44824; 13, V. carchariae 5732; 14, K. pneumoniae 44824; 15, V. parahaemolyticus 5474; 16, negative control (distilled water).