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. 2015 Jul 21;6:7715. doi: 10.1038/ncomms8715

Figure 5. Effects of biliary diversion on liver histology and hepatic gene expression.

Figure 5

(a) Liver steatosis as assessed by H&E staining in lean, DIO, GB-D, GB-J, GB-IL and RYGB mice at 8 weeks after surgery was significantly reduced (b) in GB-IL and RYGB mice relative to DIO. (c) Expression of FXR target genes in liver at 4 weeks post-operative. (d,e) Canonical pathways most differentially expressed between GB-IL versus DIO (d) and RYGB versus DIO (e). Expression of hepatic genes involved in (f) agranulocyte adhesion, (g) cholesterol biosynthesis, (h) eicosanoid signalling and (i) stellate cell activation. (j) Inflammation by hepatic F4/80, (k) Ki-67 and (l) caspase 3 by immunohistochemical staining. *P<0.05; **P<0.01, ***P<0.001 versus DIO by one-way analysis of variance with Dunn's post-test. Values are mean±s.e.m. (N of 5–10 per group for b–i; 4 per group for j–l). Magnification bar, 200 μm.