Table 1. Downregulation of phosphorylation of distinct proteins after LPS+Dex treatment and MSK1 inhibition determined by phospho-array.
| Gene name | Target | Phospho-expression | Fold change |
|---|---|---|---|
| Ccnd3 | Cyclin D3 (p-Thr283) | Down | 0.694 |
| Crk | CrkII (p-Tyr221) | Down | 0.681 |
| Hdac1 | HDA C1 (p-Ser421) | Down | 0.545 |
| Hsp90ab1 | HSP90B (p-Ser226) | Down | 0.664 |
| Hspb2 | HSP27 (p-Ser82) | Down | 0.723 |
| Kdr | VEGFR2 (p-Tyr951) | Down | 0.613 |
| Lyn | LYN (p-Tyr507) | Down | 0.674 |
| Nf2 | Merlin (p-Ser10) | Down | 0.557 |
| Raf1 | Raf1 (p-Ser259; p-Ser289) | Down | 0.404 |
| Rps6ka1 | P90RSK (p-Thr359/Ser363; p-Thr573) | Down | 0.626 |
| Rps6ka5 | MSK1 (p-Ser360) | Down | 0.730 |
| Src | Src (p-Tyr529) | Down | 0.641 |
| Syk | SYK (p-Tyr525) | Down | 0.697 |
| Ywhaz | 14-3-3 zeta (p-Ser58) | Down | 0.720 |
| Pgr | Progesterone receptor (A b-190) | Down | 0.756 |
| Krt18 | Keratin 18 (A b-52) | Down | 0.779 |
| Jun | c-Jun (A b-93) | Down | 0.781 |
BMDMs, bone marrow-derived macrophages; Dex, dexamethasone; LPS, lipopolysaccharide.
BMDMs were pretreated for 60 min with and without 10 μM SB747651A, and then treated with LPS+Dex for 4 h, and changes in protein phosphorylation events were measured with the Phospho Explorer Antibody Array (Full Moon BioSystems). The table lists proteins where the expression of protein phosphorylation was downregulated after MSK1 inhibition. The fold change indicates the change of downregulation.