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. 2015 Jul 16;6:7775. doi: 10.1038/ncomms8775

Figure 1. Age-dependent changes in temperature preference and brain tyrosine hydroxylase (TH) levels.

Figure 1

(a) Temperature preference of wild-type flies at different ages (7–42 days). (b) TH immunostaining in the brain of 7- (7D) and 21-day-old (21D) flies. Inset, magnified view of MB β′-tip. (c) Fluorescent intensity of TH-immunopositive signals in MB β′-tip of flies at different ages. (d) An optical section through β′-/β-lobes indicating dopamine receptor (DopR) immunostaining in 7D (left) and 21D (right) flies. Insets, magnified views of MB β′-tip (top) and β-tip (bottom). (e) Fluorescent intensity of DopR-immunopositive signals in MB β′-tip and β-tip of 7D and 21D flies. Scale bar, 20 μm. Values represent mean±s.e.m. (n=12). ***P<0.001; **P<0.01; NS, not significant.