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. 2015 Jul 21;6:7838. doi: 10.1038/ncomms8838

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(a) RAW264.7 cells were transduced with lentivirus-encoding Cas9 and guide RNA targeting murine Rnf145 (or intergenic control). Where indicated, complementary DNA rescue was performed with human RNF145 (or GFP control). Oxidative burst was monitored by luminol chemiluminescence. (b) Examples of indel mutations obtained from primary bone marrow-derived macrophages transduced with lentivirus-encoding Cas9 and guide RNA targeting Rnf145. The guide sequence is outlined in red and the PAM sequence is circled. (c) Oxidative burst in bone marrow-derived macrophages from the indicated mouse strains. (d) Oxidative burst in bone marrow-derived macrophages from Ncf4^+/− mice transduced with CRISPRs targeting Rnf145.