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. 2015 Apr 15;128(8):1475–1480. doi: 10.1242/jcs.166363

Fig. 4.

Fig. 4.

Exogenously expressed Hsp90β rescues endogenous Hsp90β-downegulated HDF motility.(A) HDFs with control sh-LacZ (bars 1–3), Hsp90β downregulation (bars 4–6), Hsp90β downregulation and re-expression (bars 7–9) or Hsp90β-downregulation and Hsp90α re-expression (bars 10–12) were subjected to the migration assay in response to the stimulations indicated. A quantification of the migration index for the cells is shown (MI, %). Results are mean±s.e.m., n = 3. *P<0.05 (Student's t-test). (B) Overexpression of LRP-1-II in Hsp90β-downregulated cells (Sh-90β + LRP-1-II). (C) LRP-1-II rescues the motility defect of Hsp90β-downregulated cells in response to extracellular Hsp90α stimulation. (D) A schematic of the proposed method of Hsp90α and Hsp90β action. When tissue is damaged, acute hypoxia triggers cells in the wound edge to secrete Hsp90α. Hsp90β stabilizes LRP-1 and the secreted Hsp90α binds and signal through the LRP-1 receptor to promote cell migration and wound healing.