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. 2015 Apr 15;128(8):1494–1506. doi: 10.1242/jcs.161554

Fig. 4.

Fig. 4.

Interaction between 9-1-1 complex and Zip3 protein.Interaction between 9-1-1 complex and Zip3 protein. (A) Immunoprecipitation (IP) of Ddc1–Flag from mec3 (non-tag), DDC1-Flag, and RAD17-HA DDC1-Flag cells. Whole-cell extracts (WCE) and immunoprecipitation products were probed with anti-Mec3, anti-Flag and anti-HA antibodies. (B) Pulldown assay of FATT(-Myc)-Zip3. E. coli lysates expressing either FATT–Zip3 or FATT–GFP were incubated with magnetic beads coated with anti-c-Myc antibodies. The beads (Input) were incubated with yeast meiotic cell lysates (at 4 hours) from the wild-type, RAD17-HA DDC1-Flag and mec3 (non-tag) deletion cells. The eluates were analyzed by western blotting using anti-Mec3, anti-c-Myc, anti-Flag or anti-HA antibodies. Anti-Flag antibody can detect both Ddc1–Flag and FATT–Zip3–Flag. The asterisk indicates a non-specific band.