Figure S1.

Expression Levels and Developmental Phenotypes of chico Genomic Rescue Constructs, Related to Figure 1

(A) Log-transformed levels of chico mRNA relative to actin in females of the indicated genotypes. chico expression was significantly reduced in chico mutants (p < 0.05, t test compared to WT controls), but not in the presence of any of the genomic rescue constructs. Means ± SEM.

(B–D) The PI3K-binding site, but not the Grb2/Drk-binding site, of Chico is essential for female sterility (B) and increased metabolic stores (C and D). Means ± SEM, ^∗∗p < 0.05, t test (compared to WT controls).