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Site selective oxidation with PikCD50ND176QE246A-RhFRED. C–O bonds highlighted in red = hydroxyl group introduced in the P450 reaction. Numbers given in parentheses are TTN values determined in triplicate. Reaction conditions: 1 mM substrate, 5 µM enzyme, 1 mM NADP+, 5 mM glucose-6-phosphate, 1 U/mL glucose-6-phosphate dehydrogenase.
