Fig.3.

Effect of eicosapentaenoic acid (EPA) incubation time on mRNA expression levels of PPARγ and CYP-19. GCs, after serum starvation, were incubated in 100 ng/mL follicle stimulating hormone (FSH) alone or in combination with 50 μmol/L EPA for 12 hours, 24 hours and 48 hours. Cell lysates were prepared and analyzed by real-time PCR for genes expression levels. Expression levels of PPARγ (A) and CYP-19 (B) in each lysate were normalized to the amount of GAPDH and represented as fold of FSH-treated control. The mean ± SD of three independent experiments with cells pooled from 5 women per group per experiment (repeated-measures ANOVA. *; P<0.05 and †; P<0.05 vs. FSH-treated control and 12-hour incubation, respectively).

PCR; Polymerase chain reaction, GCs; Granulosa cells and h; Hours.