Figure 3.

A GM-CSFR-β Chain Signal Drives Eosinophil But Not Neutrophil Accumulation and Is Required for the Development of Colitis

Colitis was induced in C57BL/6 WT mice and Csf2rb^−/− mice (A–C) or mixed BM chimeric mice (D) upon infection with Hh combined with anti-IL-10R treatment. Mice were analyzed 2–3 weeks following induction of colitis (A–D). (A) Representative staining of EoPs and frequencies of eosinophils among total BM cells. (B) Representative photomicrographs of mid-colon sections (magnification 100×) and colitis scores. (C) Representative staining and frequencies of neutrophils and eosinophils among total cLPL and ratio of activated/resting eosinophils. Data points represent individual mice and bars represent means (A–C). (D) Representative staining of cLPL from a mixed BM chimera (WT CD45.1 and Csf2rb^−/− CD45.2). Frequencies ± SD (n = 6), gating and statistics are indicated. Data are representative of at least two independent experiments. See also Figure S3.