Table 1.

All primers and probes used in the study.

Primer (5′–3′)	Sequence	Expected size in VCS1703A (BP)	Reference
Cc	TCGGTACCGAGTATTTCTACCCAACACCT	783	La Fontaine et al. (1993)
Ac	CGGGGTTATGTAGCTTGC	783	La Fontaine et al. (1993)
27F	AGAGTTTGATCMTGGCTCAG	1500	Lane (1991); Baker et al. (2003)
1525R	AAGGAGGTGWTCCARCC	1500	Lane (1991); Baker et al. (2003)
pgrAF1	CCTGCACCATGCTTGTTAAA	290	Calvo-Bado et al. (2011a)
pgrAR1	GCTGTTGGTGGTTTGGCTAT	290	Calvo-Bado et al. (2011a)
M13F	GTAAAACGACGGCCAG	N/A	Supplied in the cloning kit
M13R	CAGGAAACAGCTATGAC	N/A	Supplied in the cloning kit
DNTR02F	(FAM)-GATCCATCGTTTCATCGTCA	549	Russell et al. (2014)
DNTR02R	CGCACTTTAGCCGTTATGTTT	549	Russell et al. (2014)
DNTR09F	(VIC)-GGCGTAAACGAAATGCCTAA	987	Russell et al. (2014)
DNTR09R	ATCGGCGGAAGATTGTCTC	987	Russell et al. (2014)
DNTR10F	(NED)-CCGTCTATCCACCCGATTTA	626	Russell et al. (2014)
DNTR10R	TTGAACCGCGTCACTATCAG	626	Russell et al. (2014)
DNTR19F	(PET)-CCCGTCGAATCACTCCAG	854	Russell et al. (2014)
DNTR19R	GGTAGCGCCGAAGAAAGA	854	Russell et al. (2014)
rpoDF	GCTCCCATTTCGCGCATAT	61	Calvo-Bado et al. (2011b)
rpoDR	CTGATGCAGAAGTCGGTAGAACA	61	Calvo-Bado et al. (2011b)
rpoD Taqman probe	(6FAM)-CATTCTTACCGGKCG-(BBQ)	61	Calvo-Bado et al. (2011b)
pgrAF	CATGAATGATAATATTTACCTTTTCGTT	298
pgrAR	AAGATTGATGATGCTCCAGAAGAAG	298
pgrA Taqman probe	(6FAM)-CCTGCACCATGCTTGTTAAACTCT AATTTT-(BBQ)	298
pgrBF	AAAGGTGATCTCAACTGTATCGTCAT	N/A
pgrBR	AATYARCARMGCCARAATTAGAGCTTAAT	N/A
pgrB Taqman probe	(6FAM)-TTTACCCGCACCGTKCT-(BBQ)	N/A

FAM – Carboxyfluorescein, BBQ (Black Berry Quencher). BP is the size of fragment in base pairs.