Table 3.
Estimated Hands-on-time and Time-to-results
| KRAS LCD-array Kit | Hands-on-time in minutes | Time-to-result in minutes |
|---|---|---|
| PCR reaction | 15 | 150 |
| Hybridization | 20 | 60 |
| Evaluation on PC | 10 | 10 |
| Overall | 45 | 220 |
| Sanger sequencing | ||
| ᅟPCR reaction | 15 | 150 |
| ᅟGel preparation and electrophoresis | 25 | 45 |
| ᅟDucumentation and Evaluation of Dilution | 10 | 10 |
| ᅟEthanolprecipitation and centrifugation | 20 | 35 |
| ᅟElution of the Pellets in pure water | 10 | 10 |
| ᅟSequencing reaction | 10 | 160 |
| ᅟAdd purification beads and Incubation | 10 | 40 |
| ᅟSequencing run for all exons (forward and reverse) | 10 | 250 |
| ᅟEvaluation on PC | 10 | 15 |
| ᅟOverall | 120 | 715 |
| ᅟMALDI MS | ||
| ᅟPCR reaction | 15 | 150 |
| ᅟDephosphorylation SAP reaction | 5 | 60 |
| ᅟCycled MassEXTEND reaction | 10 | 150 |
| ᅟSample conditioning, Nanodispensing | 10 | 40 |
| ᅟMS analysis and calling | 5 | 30 |
| ᅟOverall | 45 | 430 |
| ᅟNGS | ||
| ᅟLibrary Preparation | 30 | 300 |
| ᅟEmulsion PCR | 10 | 360 |
| ᅟSequencing | 20 | 300 |
| ᅟData analysis | 10 | 60 |
| ᅟOverall | 70 | 1020 |