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. 2015 Jul 30;48(1):42. doi: 10.1186/s40659-015-0034-7

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© Takeyama et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Angiogenesis assay measuring tube formation ability of HUVECs with conditioned medium from ARPE-19 cells. a Representative photomicrographs. Typical tube formation was not seen in any of the samples with conditioned medium collected from cells cultured at temperatures from 37 to 31°C after 24 and 48 h. Significant differences were observed in the number of branching points and total skeleton length (arrows) among each of the conditions. Bar 50 μm. b Quantitative analysis of the number of branching points and total skeleton length. Data are presented as mean ± SD (n = 4). *P < 0.05, **P < 0.01.