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. 2015 Jul 28;21(1):62–70. doi: 10.1038/mp.2015.101

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Copyright © 2016 Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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Other transcription factors tested. (a–h) Immunostaining of MRC5 cells reprogrammed with Ascl1, FoxA2, Lmx1b, FEV and p53 shRNA (AFLbVp) (a), Ascl1, FoxA2, Lmx1a, FEV and p53 shRNA (AFLaVp) (b), AFLbVp plus NeuroD1 (c), AFLbVp plus NeuroD2 (d), AFLbVp plus miR124 (e), AFLbVp plus NKX2.2 (f), AFLbVp plus Nurr1 (g) or AFLbVp plus Pitx3 (h). (i–l) Quantification of the percentage of 5HT⁺ neurons or Tuj1⁺ neurons among all cells (DAPI⁺) (i) or the average number of 5HT⁺ (j), Tuj1⁺ (k) or DAPI⁺ (l) cells per view. *^{, #}P<0.05, vs 5HT⁺ or Tuj1⁺ for AFLbVp, respectively, cells from at least five random views in each of the three wells from three independent experiments were counted. Bars=50 μm.