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. Author manuscript; available in PMC: 2015 Jul 29.
Published in final edited form as: Dev Dyn. 2007 Nov;236(11):3077–3087. doi: 10.1002/dvdy.21354

Fig. 7.

Fig. 7

Multisite Gateway cloning to generate a tissue-specific Tol2 construct for expression of C-terminal fusion proteins. A: Three-way multisite reaction into pDestTol2pA. The egfp and mcherry Entry clones are the same as those used in Figure 4. Note that att sites are not to scale. B–G: Embryos injected with 25 pg transposase mRNA and 25 pg pTolbactin2:egfp-mcherry; lateral view, dorsal is up, anterior to the left. B: Transmitted light illumination. C: Illumination to visualize green fluorescence. D: Illumination to visualize red fluorescence. E–G: Confocal micrographs; embryo is different than the one pictured in B. E: Mosaic green fluorescence of Egfp-mCherry observed in epidermal cells (arrows) and muscle cells (arrowheads). F: Red fluorescence of Egfp-mCherry. G: Overlay of images in E,F.