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Karyotypes of Hoplias intermedius and Hoplias brasiliensis arranged from Giemsa-stained (a, f) and C-banded (b, g) chromosomes. Sequentially DAPI- (c, h) and CMA3-(d, i) stained metaphase chromosomes of both species documenting the GC-rich positive heterochromatic blocks (arrowed). Images from H. intermedius and H. brasiliensis are represented in (e) and (j), respectively. Bar = 5 μm
