Fig. 4.

FGF21 suppressed CHOP expression induced by ox-LDL and 7-KC in macrophages. CHOP mRNA level was tested by Real-time PCR in THP1 derived macrophages treated by ox-LDL (a) or 7-KC (b) in the presence or absence of FGF21 for 24 h, and 18S rRNA was used as internal control. c CHOP protein level was tested by western blotting. d Quantification of the results in c. DR5 mRNA level was measured by Real-time PCR in THP1 derived macrophages treated by ox-LDL (e) or 7-KC (f) in the presence or absence of FGF21. 18S rRNA was used as internal control. Data are presented as the mean ± S.E. of at least three independent experiments. *, p < 0.05, **, p < 0.01. N.S, no significance