Fig. 1.

Composition of the challenge medium influences extent and kinetic of pathogen-specific induction of TNF and NOS2A expression. a Influence of the different stimulation media upon extent and kinetics of the pathogen specific induction of TNF and NOS2A expression in pbMEC. a) Effect of absence (SM0) or presence (SM10) of 10 % FCS in the challenge medium to which 30 μg/ml of heat killed E.coli ₁₃₀₃ or S. aureus ₁₀₂₇ had been added. The mRNA concentration (ordinate) was measured at different times after challenge (abscissa). b) Same as a), but the challenge medium was supplemented with 10 mg/ml of total milk protein (SM milk) and S. aureus strain N305 was also included. Note that the addition milk increased the base line expression (control). c) Same as a), but the challenge was done with two S. aureus strains. Values are means and SEM. (error bars) from two independent biological replica, each sampled in duplicate. b Data are from the same experiments as shown in (a), but the EXPANDER software was used to display the data for several genes. Each line displays the fold changes (compared to the unstimulated control) in the expression of the respective gene as indicated over the time of the challenge (1, 3, 24 [h]), normalized across all conditions to the average of 0 and variance 1. Data have been taken from Additional file 2: Table S1A. Group A, immediate early genes, Group B, secondary immune response genes. c Same as (b), but the analysis included only the data from the two different S. aureus strains (indicated). Data have been taken from Additional file 2: Table S1B *, p < 0.05 vs. unstimulated control (t-test, with Bonferroni’s correction)