Fig. 4.

Modulation of TTP expression through FCS. a TTP expression is only activated by E. coli particles in SM10. pbMEC were stimulated with 30 μg/ml of heat killed pathogens media with or without 10 % FCS (SM10 or SM0), for the time as indicated. The fold increase in the TTP mRNA concentration above the control value is indicated. Mean values ± SEM from two biological replica experiments, each assayed in duplicate. Only values from E. coli stimulation at 1 h and 3 h in SM10 resulted in significantly increased mean values (p < 0.001). b Addition of milk prevents induction of TTP expression. Same experimental setting as above, but stimulation was in SM Milk. No significant induction of TTP mRNA concentration, but a significant (p < 0.05) decrease compared to the unstimulated control at t 1 h. c Pam2CSK4, but not LPS triggers TTP expression irrespective of the presence of FCS. Same experimental setting as in A) but stimulation was with 10 ng/ml of either Pam2CSK4 or highly purified LPS. All values, but that for LPS at 1 h after the challenge differed significantly (p < 0.01) from the control value