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. 2015 Jul 30;14:142. doi: 10.1186/s12943-015-0414-1

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© Shiba-Ishii et al. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Generation of Tg-SPC-SFN^+/−. a A cDNA for SFN was inserted between the SPC promoter and both the splicing and polyadenylation (polyA) signal sequences. b RT-PCR using cDNA extracted from lung, heart, kidney, liver, and spleen of representative Tg-SPC-SFN^+/− mice. c hSFN IHC using FFPE specimens of each of the organs from representative Tg-SPC-SFN^+/− mice. Transgene expression (hSFN) was detected only in lung. Magnification: ×200. d CT image of representative Tg-SPC-SFN^+/− lungs 10, 15, and 20 weeks after NNK administration. Arrows indicate suspected tumors. Scale bar: 10 mm. e Gross appearance of lungs of Tg-SPC-SFN^+/− mice administered NNK (1) and saline (2). Arrows indicates tumors. Scale bar: 5 mm. f hSFN IHC using FFPE specimens of tumors and adjacent normal lung from Tg-SPC-SFN^+/−mice. Magnification: ×200