Figure 2. STIM1 in T cells controls myeloid cell infiltration of Mtb-infected lungs.

(A) Immunohistochemistry of CD68 expression on monocytes/macrophages in lungs of Mtb-infected mice at 114 d.p.i. Images are representative of 5 mice per group. Original magnification, ×40 (left panels); ×400 (right panels). (B and C) Frequencies of lung myeloid cell populations at 114 d.p.i. Representative flow cytometry plots of 5 mice per group analyzed. Blue gate numbers represent CD11b^–CD11c⁺Gr1^– AM (no. 1), CD11b⁺CD11c⁺Gr1^– mDC (no. 2), CD11b⁺CD11c^–Gr1^hi neutrophils (no. 3), CD11b⁺CD11c^–Gr1^int monocytes (no. 4), and CD11b⁺CD11c^–Gr1^– RIM (no. 5). (D) Absolute numbers of myeloid cell populations in the lungs of WT and Stim1^CD4 mice (as defined in B and C) over the course of Mtb infection. Line graphs show mean ± SEM of 5 mice per group and time point. (E) Concentrations of the proinflammatory cytokine IL-1β, myeloid growth factors (GM-CSF, M-CSF), and chemokines (MCP-1/CCL2, MIP-1α/CCL3, RANTES/CCL5) in lung homogenate supernatants from Mtb-infected WT and Stim1^CD4 mice analyzed at 107 d.p.i. and 114 d.p.i. by multiplex analysis. Individual values for both days are pooled and indicated by symbols. Bar graphs show the mean of 9 mice (4 on 107 d.p.i. and 5 on 114 d.p.i.) per group. Statistical significance was calculated by Student’s t test. *P < 0.05; **P < 0.01; ***P < 0.001.