FIG 6.

Expression of TcdE₁₆₆ from a modified RBS. (A) Growth curve of the R20291::tcdE mutant complemented with TcdE₁₆₆. The strain with the vector alone was used as a control. The arrow indicates the time point at which ATc (10 ng/ml) was added to induce protein expression. (B) Clearing of the culture was observed ∼4 h after induction. (C) Membrane proteins from R20291::tcdE mutants carrying pRGL60 (TcdE WT) and pRGL297 (TcdE₁₆₆) were probed with anti-6×His antibodies to detect the TcdE WT and TcdE₁₆₆, respectively.